Page 4 - AXIMA Performance

AXIMA Performance™ -

Excellence without Compromise

High performance MS data
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90 The AXIMA Performance demonstrates high resolution
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and mass accuracy across a wide mass range, from
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pharmaceutical compounds, through peptides to high
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50 73954 mass proteins, to enable a variety of applications in the
40 research environment. A highly optimized linear mode
30 generates reproducible and sensitive results for very
20 high mass compounds and complexes, extending the
10 array of sample classes that may be analyzed. A typical
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50000 100000 150000 200000 example of an intact high mass protein,
m/z
Immunoglobulin G, is shown (left).
Linear MS spectrum of Immunoglobulin G
Powerful precursor ion selection

The revolutionary monoPULSE ion gate allows the
selection of MS/MS precursors with industry-leading
resolution. Ions from complex mixtures or closely
associated neighbouring isotopic envelopes may be
isolated and subsequently fragmented. The gate
resolution of greater than 400 (FWHM) readily permits
the analysis of peptides with similar nominal mass,
even with overlapping isotopic distributions.

Efficient MS/MS from
high-energy CID

Once selected for MS/MS fragmentation, precursor
ions are subjected to true high-energy collisions with
the chosen collision gas, e.g. helium. The resulting
fragment ions are analyzed in the second TOF region
incorporating the curved field reflectron and detected
with the ultrafast MCP detector.
The high sensitivity means that good quality, usable
MS/MS spectra can often be obtained for low sample
amounts.
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The novel additional Low Mass Zoom (LMZ) scan allows
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focus of the analysis to be directed around the low
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mass region of the MS/MS spectrum. This feature can
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be employed to increase confidence in MS/MS based
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quantitation experiments and to aid in de novo
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sequencing of unknowns by enhancing the immonium
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ion region of the spectrum.
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100 200 300 400 500 600 700 800 900 1000
m/z
Typical high-energy CID MS/MS spectrum

AXIMA Performance