Page 5 - Microorganism Species Analysis

Microscope Microscope
Morphology
Microorganism Solutions Microorganism Solutions

Microscope Observations of Microorganisms Data
Cultivation, staining, and microscope observations are widely used for the identification of microorganisms. Generally,
observations are performed by optical microscopy combined with staining. However, the detailed morphological features of Scanning Probe Microscope Observations of Microorganisms
bacteria and other micron-order microorganisms are difficult to observe at the resolution level of optical microscopes.
Some scanning probe microscope observations of microorganisms are shown below. The scanning probe microscope Microorganism Species Observation of
Shimadzu employs a scanning probe microscope to capture more detailed morphological features of bacteria to better meet
offers high-resolution images of microorganisms that cannot be observed by an optical microscope. It requires very
the requirements for microorganism observations.
simple pretreatment: dripping the bacteria on to a glass sheet, adsorption of the sample, and air-drying only. It clearly
Observation of
captures the detailed morphological features of the bacteria shapes and flagella.
Microorganism Species

Features of the Scanning Probe Microscope E. coli Lateral flagella of marine bacteria Lactobacillus community

The scanning probe microscope permits high-magnification observations in air up to several 10,000x magnification Microorganism Species Identification of
without staining or other pretreatment of the sample surface.
The electron optical system with excellent low-acceleration voltage characteristics is suitable for shape observations
of the sample surface.
Identification of
Microorganism Species

The rod-shaped bacteria and flagella are This image shows a bacteria culture This image shows lactobacillus contained Specific Microorganisms Detection of
clearly visible. (Vibrio alginolyticus 9) adsorbed on a in commercially available yoghurt. Two
Detection of
cover glass in the solution and air-dried. different shapes of lactobacillus are
The phase image shows the flagella at apparent: rod-shaped and spherical
Specific Microorganisms
high contrast (using a phase detection bacteria. Particle analysis software can
system). (Courtesy of Dr. Kogure, Ocean be used for the calculation and statistical
Research Institute, University of Tokyo) processing of the number of lactobacillus
with respective morphological features
Scanning Probe Microscope within the field of view to obtain clear
morphological images.

The following URL shows many other scanning probe microscope images. Components Analysis of Microorganism-Derived
Components
http://www.shimadzu.com/products/lab/surface/spmd.html

Analysis of Microorganism-Derived
Fixing and drying

Bacteria SPM-9600
Scanning Probe Microscope Other Applications

Other Applications

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