Page 3 - Microorganism Species Analysis
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              Total Solutions for Microorganism Analysis and Testingg

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              Shimadzu Supports Everybody Working with Microorganisms
              While we cannot easily see microorganisms, they are closely linked to our lives. From ancient times, microorganisms have expanded   Microorganisms are researched and utilized for a wide range of purposes in many research and industry fields.
              the human diet by providing fermented foods including alcohol and pickles. In recent years, microorganisms have become    The major purposes are inspection and control, identification, searching for new microorganisms, functional investigations, and
              indispensable in the production of useful substances, such as antibiotics, taste components, and vitamins. Microorganisms are also   industrial applications.
              essential for environmental sustainability and improvements, including wastewater treatment, soil cleanup, and atmospheric balance.
              Moreover, the application of microorganisms has infinitely extended the possibilities of biotechnologies, which have undergone
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              explosive development since the 1970s. The application of microorganisms also promises solutions to food and energy problems,   R Resear r c h aims in resear r c h and industr r y fields
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              which are the biggest issues facing mankind.
                                                                                                                                         Clinical research, hygiene   Identification and detection  Pharmaceuticals  Searching for new microorganisms
              However, microorganisms can also pose a threat to people. For example, E. coli O157 bacteria that causes food poisoning, norovirus
                                                                                                                                         Chemistry         Inspection and control (pollution countermeasure)    Functional investigations/improvements
              epidemics, and bacterial contamination of food factories all present serious social problems. In addition, new antibiotic-resistant
              bacteria such as Methicillin-Resistant Staphylococcus Aureus (MRSA) and emerging viruses present significant medical problems.               Detection (investigation of biodegradation, etc.)    Extraction of antibiotics and other useful components
              To gain knowledge of microorganisms, diverse analysis technologies are applied to determine not only how many of what bacteria               Extraction of enzymes and other useful components    Inspection and control (pollution countermeasures)
              are where (identification and quantitation) but also for research into the response to bacterial components, bacterial-derived   Energy      Searching for new microorganisms       Foods         Fermentation and brewing for food production
              components, metabolites, and the environment.                                                                              Environment       Functional investigations, functional improvements   Detection (disposition kinetics)
              Shimadzu provides an extensive product range, expertise, and solutions to anyone dealing with microorganisms in a diverse range
                                                                                                                                                           Analysis of metabolites                              Inspection and control (pollution countermeasures)
              of fields, including medicine, foods, chemicals, energy, the environment, clinical medicine, and hygiene.
                                                                                                                                                           Inspection and control                               Functional investigations
                                                                                                                                                           Recovery of metabolites                              Searching for new microorganisms
                          Observation and Identification                                Detection of Specific
                                                                                                                                        Research into microorganisms is multifaceted, from the observation of microorganisms to the chemical analysis of
                                 of Microorganisms                                         Microorganisms
                                                                                                                                        microorganism-derived components.
                                                                                                                                        Consequently, the most important point is the selection of the optimal research approach and the best instruments to support the
                                                                                                                                        research. This document introduces examples of the application of Shimadzu products to these research aims.
                                                                                                                                          Workflow for microorganism research
                                                                            Ampdirect Plus                                                                                                                      Component
                                                                                                                                             Observation             Identification        Detection                                Other applications
                             DeNOVA-5000HT                                                                                                                                                                        analysis
                                                   AXIMA series
                                                                                                  Norovirus G1/G2
                                                                                                 detection reagent kit
                                                                  Basic               MultiNA                                             Contents
                                                               research to
                      BioSpec-nano                                applied
                                          SPM-9600                                                                                                   Aims                   Target                            Analytical Instrument/Reagent  Page
                                                                 research
                                                                                                                                                     Observation of         Morphology                        Microscope                     P. 4
                                                                                                                                                     Microorganism Species
                                                                                                                                                     Identification of      Genome Information                DeNOVA-5000HT DNA Sequencer    P. 6
                                                                                                                                                     Microorganism Species  Low-Molecular Information         LCMS-2020                      P. 8
                                                                                                                                                     Detection of Specific   Nucleic Acid (DNA)               Ampdirect Plus                 P. 10
                                                                                                                                                     Microorganisms         Nucleic Acid (RNA)                Norovirus G1/G2 Detection Reagent Kit  P. 12
                                                                                                                                                     Analysis of            Protein, Peptide, Lipid, etc.     MALDI-TOF MS                   P. 14
                                                                                                                                                     Microorganism-Derived   Protein                          MALDI-TOF MS                   P. 18
                                                                                                                                                     Components             Amino Acid, Fatty Acid, Organic Acid, etc.  GCMS-QP2010 Plus     P. 20
                                                                                                                                                                            Saccharide, Organic Acid, Amino Acid, etc.  Prominence HPLC      P. 22
                                       Prominence UFLC            LCMS-2020              GCMS-QP2010 Plus                                                                   Amino Acid, Organic Acid, and Aflatoxin  Prominence UFLC         P. 24
                                                                                                                                                                            Nucleic Acid, Amino Acid, and Aflatoxin  LCMS-2020               P. 26
                                               Analysis of Microorganism Components                                                                  Other Applications     Protein, Peptide, etc.            Spectrophotometers             P. 30
                                                                                                                                                                            Metal                             AA-7000 Series, etc.           P. 31
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