Page 4 - Shimadzu PPSQ-51A-53A

Analytical Stability and Higher Detection

Baseline Stability Retention Time Reproducibility
PPSQ series protein sequencers separate Isocratic sequence analysis provides more stable
PTH-amino acids isocratically. This improves retention times. Therefore, peaks detected in
baseline stability and allows high-sensitivity previous cycles can be cancelled using
analysis of PTH-amino acids. substation chromatogram processing, making it
easier to identify sequences.

PTH-AA 10pmol
N
D E Q H
S T G A Y
R M V W
P K I
F L

Higher Detection
Cycle 10 PTH-Val
PPSQ-51A/53A PPSQ-31B/33B

Cycle 21 PTH-Ile
PPSQ-51A/53A PPSQ-31B/33B

Sample: Horse myoglobin 10pmol
Results show the subtraction chromatograms by SPD-M30A (PPSQ-51A/53A) and SPD-20A (PPSQ-31B/33B) connected tandemly after
Edman degradation.

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