Page 13 - Shimadzu Nexera Series

High-Sensitivity Impurity Analysis

The SPD-M40 detector achieves an mAU Ketoprofen Abs（AU）
extremely high level of sensitivity and 2.50 R 2 =0.9998
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linearity (up to 2.5 AU). This allows Area% 0.12 %
0.56 %RSD (Area)
quantitation of very lowconcentration 4 Impurity A
Area% 0.045%
impurities even in high-concentration 3 0.96 %RSD (Area)
samples. The UV cut-off filter installed in 2 Impurity C
the detector prevents sample degradation
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due to UV light, helping to maintain good
0 2.00 2.25 2.50 2.75 3.00 3.25 3.50 0 0 100 200 300 400 500 600 700 800 Conc.(mg/L)
linearity at low concentrations.
Example: Ketoprofen analysis reveals impurities of Detector linearity for ketoprofen
area% < 0.2%
Reduced Stray Light and Improved Measurement Circuits (Patent Pending)
With the SPD-M40, the measurement circuits have been improved, and the amount of stray light in the optical system, a cause for linearity
errors, has been reduced (to less than 1/3 of the usual amount). As a result, the absorbance linearity has been improved to at least 2.5 AU.

Ultra-low Carryover

The Nexera boasts ultra-low carryover, even on a high-sensitivity LC/MS/MS. This reduces time spent on rinsing, resulting in a shorter
overall analysis time.

(x10,000)
3.0
Elimination of Dead (x1,000)
2.5 3.0
Volume (Patent Pending) Chlorhexidine 500 ng/µL
2.0 2.5
Dead volume in the flow line 1.5 Blank 2.0
will lead to carryover from 1.0 1.5 Carry over
sample residue. In the SIL-40 0.5 1.0 0.00007%
series, the sample loop and 0.0 0.5
0.0 0.5 1.0 min
needle are connected linearly, 0.0
eliminating dead volume, and 0.30 0.6 0.9 min
reducing carryover. Rinse Conditions: Normal
After analysis of Chlorhexidine, the carryover to a blank solution is negligible.

Stable Baseline

Baseline fluctuations can affect peak area calculation, reducing the accuracy of quantitative results. The SPD-M40 photodiode array
detector’s “Advanced TC-Optics” function adjusts the temperature of the flow cell, lamp, and optical system to lessen the impact of
external temperature changes. Noise and drift have also been reduced by 40% compared to the previous model.

Room Temp. Area %RSD
25°C (n=20)
20°C
mAU
2.5 SPD-M40
0 0.62% Higher reproducibility
−2.5
−5.0
−7.5
−10.0
−12.5 Another Vendor's
PDA Detector 1.87%
0 50 100 150 200 250 min

Advanced TC-Optics (SPD-M40) (Patent Pending)
With the SPD-M40, in addition to conventional cell temperature regulation, the lamp and spectrometer, which are responsible for thermal
effects on absorbance, are also thermally regulated, further reducing baseline fluctuations caused by the light source.

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