Page 5 - Shimadzu GC-2010 Plus

Advanced Flow Technology

Multi-Switching Analysis of Essential Oil
1st Column : MEGA SE-52 0.25 x 25 m df=0.25 μm
Oven temp : 50 °C - 280 °C (3 °C/min)
Injector : 250 °C
Split ratio : 1 : 100
Monitor FID : 290 °C
Advanced Flow Technology is Shimadzu´s solution to provide enhanced separation H2: 50 mL/min, Air: 400 mL/min, Make-up: 0 mL/min
power and operational efficiency for applications with complex sample matrices. Switching : 8 times
This enhanced capability is based on the high precision Advanced Flow Control 2nd Column : MEGA DetTBuSililBeta 0.25 x 25 m df=0.25 μm
Oven temp : 45 °C (12.00 min) - 180 °C (2 °C/min)
(AFC) of the GC-2010 Plus.

Advanced Flow Technology for high-performance separation
1st GC Chromatogram
8 compounds are switched onto the 2nd column.
Multi-dimensional
2nd GC Chromatogram
GC/GCMS System
8 compounds are separated into 15 peaks.
MDGC/GCMS-2010
Data by
Universita degli Studi di Messina
Prof. Luigi Mondello
Alessandro Casilli
Peter Quinto Tranchida
Danilo Sciarrone

Chromatogram Obtained with 1 st Column Reproducibility of 1st Column
Chromatogram
Area Retention Time
Heart-cut positions
Compound MeOH tAmOH MeOH tAmOH
1 174308 435368 3.008 12.712
2 171031 427495 3.009 12.712
3 173117 433085 3.009 12.712
A multi-dimensional GC/GCMS system performs separations using two columns that have Without 4 174715 438180 3.009 12.712
Switching heart-cutting
different chromatographic selectivity. When components of interest are insufficiently AuxPress 5 172315 430744 3.008 12.712
flow diagram Monitor Detector
separated on the first column, they can be selectively introduced ("heart-cut") to a second 6 174633 436304 3.008 12.712
chromatographic column with different selectivity. This enables enhanced chromatographic With 7 175269 439073 3.009 12.712
separation that cannot be attained in conventional single-column analysis. In addition, the Detector 2 nd 1 st heart-cutting 8 175863 441410 3.008 12.711
precise flow-switching technology, which is supported by a high-precision digital flow 9 172717 430224 3.008 12.712
controller, ensures heart-cut analysis with the high level of reproducibility demanded of 10 172002 435496 3.008 12.712
Flow diagram of Standby mode 173597 434738 3.008 12.712
complex capillary GC separations. Average
AuxPress
STDEV 1582 4347 0.00052 0.00032
Monitor Detector
CV(%) 0.912 1.000 0.017 0.002
Detector
2 nd 1 st Chromatogram Obtained with 2 nd Column Reproducibility of 2nd Column
Area Retention Time
Flow diagram of Cut mode Compound Acetone IPA ETBE Bz TAME nBuOH Acetone IPA ETBE Bz TAME nBuOH
1 241902 258122 322917 584033 302735 341100 5.515 5.880 11.563 16.501 18.137 18.733
2 237073 253283 316835 572472 296948 334684 5.516 5.880 11.565 16.501 18.136 18.734
• Standby Mode • Cut Mode Multi-Deans’ Switching 3 240294 256361 321149 580369 301337 339373 5.516 5.881 11.565 16.503 18.138 18.733
In the past, multidimensional GC has been 4 242492 259231 324511 587335 304680 343516 5.515 5.881 11.565 16.502 18.136 18.732
accomplished using a switching mechanism known as 5 238727 254946 318735 576768 299044 337067 5.515 5.880 11.563 16.500 18.136 18.733
Deans’ Switch. However, this system results in such 6 242091 258606 323204 584763 303246 342028 5.515 5.880 11.563 16.503 18.137 18.732
problems as a reduced recovery (sample loss) and 7 243402 260304 325211 588083 305140 344235 5.515 5.880 11.563 16.502 18.135 18.731
fluctuations in retention time after column switching. 8 241572 260384 326286 588626 301903 337481 5.515 5.880 11.563 16.501 18.135 18.732
The MDGC/GCMS-2010 system incorporates multi- 9 239256 255374 319324 576796 299153 336898 5.515 5.880 11.563 16.501 18.135 18.733
Deans switching, a new mechanism that significantly 10 238508 254885 319167 576955 299617 337750 5.515 5.880 11.564 16.502 18.137 18.730
reduces the likelihood of fluctuations in the retention Average 240532 257150 321734 581620 301380 339413 5.515 5.880 11.564 16.502 18.136 18.732
times of components eluted after column switching, STDEV 2067.56 2506.10 3159.04 5706.12 2662.17 3170.38 0.00037 0.00049 0.00067 0.00076 0.00108 0.00106
even when column switching is performed several times. CV 0.860 0.975 0.982 0.981 0.883 0.934 0.007 0.008 0.006 0.005 0.006 0.006

4 5