The potential adsorption of an analyte onto wetted surfaces of UHPLC instruments poses some critical challenges when analyzing biomolecules. While elevated pressure tolerance is required to achieve optimal chromatographic separation when using small particle size columns the inertness of the wetted surfaces is also of the utmost importance, as is resistance to corrosion due to the use of mobile phases with high salt concentrations and extreme pH values. The Nexera XS inert system offers the ideal solution for the separation of biomolecules by combining the elevated pressure tolerance of a UHPLC system with complete inertness of the sample flow path, ensured by the absence of wetted metal surfaces and offering ultra-high resistance to corrosion.
Unconstrained Recovery and Sensitivity
For the quantitative analysis of biomolecules, particularly when high sensitivity is required, the adsorption of the target compound to a metal surface can result in a dramatic decrease in sensitivity. The Nexera XS inert system prevents peak tailing and sensitivity loss by eliminating adsorption of proteins, nucleic acids, and other components to metal surfaces. As a result, reliable and accurate quantitation is possible, even for low-concentration samples.
The Nexera XS inert can detect oligonucleic acids with a strong tendency of metal adsorption.
Clear Resolution without Restrictions
The Nexera XS inert system is equipped with unique technology that ensures the complete inertness of the sample flow path. The system provides excellent peak shape and unsurpassed chromatographic separation by effectively inhibiting the adsorption of target compounds to internal surfaces.
Assured Reliability and Reproducibility
Consistent Analysis Reproducibility
In order to reduce the effect of adsoprtion of metal-sensitive compounds, a system is often passivated by repeatedly injecting samples that contain the target compounds before starting data acquisition. However, this approach not only wastes valuable samples and time, but can make it extremely difficult to acquire reliable data due to changes in the state of passivation during continuous analysis. Nexera XS inert systems eliminate the need for the preliminary passivation and provide highly reliable data from the first injection and throughout the analytical session.
Improves Quantitative Analysis Performance
Adsorption of target compounds can be especially detrimental in the low-concentration region, and can worsen calibration curve linearity. The Nexera XS inert achieves a wider dynamic range by strongly increasing the quantitative accuracy and detection of low-concentration samples.
