Page 6 - Food&Beverages Solutions-for-EtO-Analysis-SSI

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1.1 Sesame Seeds Analysis - (Application Note)

Application Databook

EtO & 2-CE Analysis in Sesame Seeds

¿ This section deals with various approaches, extraction process & different instrument
techniques used for the analysis of EtO and 2-CE in sesame seeds sample

APPROACH MODE OF ANALYSIS INJECTOR INSTRUMENT TECHNIQUE

EURL-SRM Analytical Observation Liquid SPL AOC-20i/s - GCMS-TQ8050 NX
Report (EtO & 2-CE)
st
Headspace – Method 1 (EtO & 2-CE) Dynamic Headspace SPL HS-20 NX - GCMS-TQ8050 NX
nd
Headspace – Method 2 (2-CE) Dynamic Headspace SPL HS-20 NX - GCMS-TQ8050 NX
rd
Headspace – Method 3 (EtO) Dynamic Headspace SPL HS-20 NX - GCMS-TQ8050 NX

SCAN & DOWNLOAD

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Published Application Note
document_file/applications/application_note/16786/an_06-saip-gc-031-en.pdf

st
Ø Headspace – Method 1 (EtO & 2 CE): Solvent extraction followed by the analysis with dynamic
headspace mode. For simultaneous analysis of EtO & 2 CE in different matrices, above
mentioned method is used most of the time.

Trace level quantitation of Ethylene Oxide (EtO) and 2-Choloroethanol (2-CE) in sesame seeds by using
various GCMS/MS techniques with their own merits and demerits

The European Chemical Agency (ECHA) has classified EtO in category 1B as regards carcinogenicity,
mutagenicity and reproductive toxicity, and in category 3 as regards the acute toxicity. The US National
Institute of Health (NIH) classified EtO as “known to be a human carcinogen based on sufficient evidence of
carcinogenicity from studies in humans, including epidemiological studies and studies on mechanisms of
carcinogenesis.” The US Environmental Protection Agency (EPA) has concluded that EtO is carcinogenic to
humans by the inhalation route of exposure. 2-CE is prominent metabolite of EtO and is equally hazardous
compound. EU-MRLS (Maximum Residue Levels as per European Commission) for EtO & 2-CE are different
for different commodities. Out of many matrices EU-MRL for cereals, pulses & vegetables are the lowest and
that is 0.02 mg/kg. Considering carcinogenicity and no acceptable threshold for exposure, no Acceptable
Daily Intake (ADI) was established for EtO & 2-CE and hence it is very important to quantitate EtO & 2-CE, as
low as possible in the food matrices.

With reference to EURL-SRM Analytical Observation Report, Shimadzu have successfully developed and
validated methods for trace level quantification of EtO & 2-CE impurities in sesame seeds by using Shimadzu
GCMS-TQ8050 NX with AOC-20i and AOC-20s liquid auto sampler / HS-20 NX headspace sampler (Dynamic)

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