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Introduction to Beer Analysis                                                                                             Top Beer Analyses






                    In many philosophies, the classical four elements are earth,   countries on 6 continents.                                 In the average brewery, the brewing chemist needs to   Beyond these required analyses, there is great interest in other
                    water, air, and fire. Analogous to this history is beer, which is   Some of the larger organizations with specific testing for beer   understand and address many quality assurance standards.   beer analytics. The regional water quality is known to greatly
                    made with four classical “elements”: water, barley malt, yeast,   include the American Society of Brewing Chemists (ABSC) and   Some of the major concerns are color, IBU (International   affect the flavor profile of beer, and when brews that were
                    and hops. Many varieties of beer are made using these   the European Brewing Convention (EBC). The four “elements”        Bittering Units), and alcohol content. The alcohol content on   once consistent become troublesome, examination of the water
                    ingredients, with barley malt or other grain sources are used in   in beer should be tested for characteristics, quality, and   the label is required by legal organizations such as the USA’s   can be enlightening. In this section, equipment for examining
                    the brewing process. For flavoring, hops and other additives   contamination to ensure the final product meets expectations.   Alcohol and Tobacco Tax and Trade Bureau (TBB). At some   these factors affecting beer quality and production will be
                    enhance the taste. Brewer’s yeast, typically Saccharomyces (from   Beer brewing may appear simple with only four “elements” but   point, calorie and carbohydrates content may also be required   detailed. For more information about any of these products,
                    the Greek as sugar fungus) ferments the sugars, extracted from   the chemistry is quite complex, requiring many types of   on alcohol labels.                                please contact your Shimadzu representative.
                    the grain, into ethanol and carbon dioxide.        analytical instruments to obtain the desired appearance, aroma,
                    Brewing of beer dates back more than 5,000 years. There is even   flavor, mouthfeel, and overall impression.
                    an ancient goddess of beer named Ninkasi. The Sumerians   This brochure contains a partial list of measureable parameters.
                    developed a hymn to her such that the recipe for beer could be   Should you require additional testing parameters please feel
                    passed down for generations. Today, “International Beer Day” is   free to contact Shimadzu.
                    the first Friday in August and is celebrated in 207 cities in 50
                                                                                                                                              Color, IBU, & Alcohol Content




















                                                                                                                                                                                                      Shimadzu UV-1800 for
                                                                                                                                                                                                      measurement of beer IBU and color




                                                                                                                                              One of the most useful pieces of equipment for a brewery’s   Convention (EBC). The strength of a beer is measured by volume
                                                                                                                                              laboratory is the spectrophotometer. American Society of   and expressed as a percentage of alcohol (the number of
                                                                                                                                              Brewing Chemists (ASBC) methods indicate at least 12   milliliters of pure alcohol in 100 ml of beer). Bitterness and color
                                                                                                                                              measurements, including beer bitterness (Beer-23) and beer   can be performed on a UV-Visible spectrophotometer with
                                                                                                                                              color (Beer-10), that can be achieved using a single piece of   built-in calculations. Alcohol is performed with a gas
                                                                                                                                              equipment. For the purpose of a novice brewery lab, the most   chromatograph (GC).
                                                                                                                                              important measurements are IBU, color, and alcohol content.  Examples of other beer and wort characteristics that can be
                                                                                                                                              IBU or international bittering units is a measure of the   measured by UV-Vis spectrophotometers include carbohydrates
                                                                                                                                              bitterness of beer, derived from the alpha acids in hops. The   (Beer-41), copper (Beer-19), diacetyl (Beer-25 D), FAN or Free
                                                                                                                                              color of beer ranges from pale to very dark and can be detected   Amino Nitrogen (Wort-12 and Beer-31), iron (Beer-18A, C),
                                                                                                                                              by the naked eye. However, a scale has been developed –   oxygen (Beer-34), protein (Beer-11),  total polyphenols
                                                                                                                                              degrees Lovibond – to define color intensity. There are   (Beer-35), sulfur dioxide (Beer-21), wort color (Wort-9), and
                                                                                                                                              conversion factors when switching between Lovibond, ASBC’s   Thiobarbituric Acid Index (TBI by Wort-21).
                                                                                                                                              Standard Reference Method (SRM), and the European Brewery


                                                                                                                                              Note: typical laboratory equipment such as chemicals, glassware, pipettes, shaker, centrifuges, etc. is not included.

                                                                                                                                                                                         Shimadzu’s Total Support for Beer Analysis
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