Page 4 - Shimadzu Renewable Fuels Industry

Monitoring the Fermentation Process in Bioethanol Production

In a bioethanol production facility, High Performance Liquid Chromatography (HPLC) is typically used to profile the carbohydrate,
alcohol, and organic acid contents of the fermentation broth. These aliphatic compounds display almost no ultraviolet absorbtion
and therefore are detected by using a differential refractive index detector.

mV Shimadzu BioEthanol Analyzer
5 hours 4
1000 19 hours ■Peaks System Features
1. DP4+, 2. DP3, 3. Maltose, 4.
34 hours Glucose, 5. Lactic acid, 6. Glycerol, 7. Bio Ethanol
50 hours Analyzer
Acetic acid, 8. Ethanol
750 • Complete turnkey startup package to
Decrease hit the ground running
of Sugars • Ease of access integrated system
500 • Quick batch function simplifies batch file creation for
Increase simplified use
of Ethanol • Space-saving small footprint to free up bench space
250 1
8 • Based on the award-winning Shimadzu Prominence-i
2 3 5 6 7 series HPLC
0 • Open rack access to the autosampler for convenient
0 5.0 10.0 15.0 20.0 min sample loading
Checking the amount of ethanol produced by the passage of • Remote monitoring for quick response to a production
fermentation time via HPLC problem
• Closed system with back flushing capability eliminates
system contamination
Introducing the Shimadzu BioEthanol • Shimadzu quality hardware, software and service support,
Analyzer with the RID-20A Refractive adding value to your operation

Index Detector

Shimadzu’s new BioEthanol Analyzer includes a complete turnkey system to analyze your important
compounds of interest. The system includes the newest generation Shimadzu HPLC: the Prominence-i isocratic
quaternary pumping system with the RID-20A Refractive Index Detector and Shimadzu’s liquid autosampler.
Shimadzu also includes the latest column technology with the CHATA™ solvent system.
Pumping System
The Prominence-i enjoys the flexibility of a quaternary pumping system, giving the analyst
additional solvent selection capability. A valve option provides up to seven solvents for
method development or system flush at shutdown, saving valuable time.

Autosampler
The i-Series autosampler features an ultra-fast (14 sec) injection cycle with ultra-low carryover
(less than 0.0025%). C models have 4-45°C temperature control

Refractive Index Detector
Inheriting the stability and extensibility that are the strengths of the Prominence series, the
new RID-20A reduces the time to stabilization after power-on dramatically by utilizing dual
temperature control for the optical system

Column Oven
The column oven uses forced air circulation for (ambient -10) to 85°C temperature
control. Ideal for bioethanol applications with a refractive index detector.

Display
Full-color LED touchscreen for front panel control with real-time chromatogram
display. Remote monitoring capability from a smartphone or tablet.

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