Page 5 - Shimadzu Shim-pack MC Series

Shim-pack MC PLONAS Series

Various molecules give an increase in the peak intensity on Nexera Mikros LC-MS/MS platform with an MC
PLONAS column.

Shim-pack MC PLONAS C18

3000 Aldosterone 359.2 > 189.3 (−) 6.0e2

2500 5.0e2
Micro
Semi-micro
2000 4.0e2

1500 3.0e2
Aldosterone 1 pg/mL

1000 2.0e2

500
1.0e2
0
0.0e0
1.0 2.0 3.0 4.0 5.0 1 2 3 4 5 (min)

Column : Shim-pack MC PLONAS C18 (0.2 mm I.D. × 100 mm L., 2.7 m)
* Semi-micro data acquired with the 2.1 mm I.D. column which has the same chemical pro le as an MC PLONAS column.
Mobile phase : A: Water, B: Methanol, gradient elution
Flow rate : 4 L/min
Temperature : 50 ºC
Injection volume : 1 L
MS detection : LCMS-8060, with Micro-ESI source, ESI (−)

Shim-pack MC PLONAS C8

1. All-trans Retinol (A)
2. Epoxy-Menaquinone-4 (epoxy-MK-4)
3. Menaquinone-4 (MK-4) 5 6
4. α-Tocopherol (E) 1
5. Epoxy-Phylloquinone (epoxy-K1) 4
6. Phylloquinone (K1) 7
7. Menaquinone-7 (MK-7)
3
2 Column : Shim-pack MC PLONAS C8
(0.2 mm I.D. × 100 mm L., 2.7 µm)
Mobile phase : A: Ammonium acetate aq.
B: Ammonium acetate in methanol,
gradient elution
Flow rate : 4 µL/min
Temperature : 50 ºC
Injection volume : 0.2 µL
MS detection : LCMS-8060, with Micro-ESI source,
0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 min ESI (−)
20 pg on column
* each peak is in full scale intensity