Page 11 - Shimadzu Nexera Mikros

Shim-pack MC PLONAS Series Shim-pack Trap Columns

Various molecules give an increase in the peak intensity on Nexera Mikros LC-MS/MS platform with an MC PLONAS column.
Trap Columns Wide Portfolio

Shim-pack MC PLONAS C18
Shim-pack MCT C18/C8
70 MPa pressure limitation and a unique modification on the column inner surface protects
3000 6.0e2 against absorption of the molecule.
Aldosterone 359.2 > 189.3 (−)
Shim-pack MCT LC18/LC8
2500 5.0e2
Micro A small-volume cartridge column with 40 MPa pressure limit. Useful in reducing the gradient
Semi-micro delay for a low flow rate.
2000 4.0e2

1500 3.0e2
Aldosterone 1 pg/mL
Shim-pack MCT series Shim-pack MCT L series (particle size 5 m)
1000
2.0e2
Part Number Bonded Phase Diameter (mm) Length (mm) Part Number Bonded Phase Diameter (mm) Length (mm)
228-59938-91 0.3 227-32702-01 Trap column holder for Shim-pack MCT L
500 C18 35
1.0e2 228-59938-92 0.5 227-32702-02 C18 0.3
5
228-59939-91 0.3 227-32702-03 C8 0.5
C8 35
0 228-59939-92 0.5
0.0e0
1.0 2.0 3.0 4.0 5.0 1 2 3 4 5 (min)
Column : Shim-pack MC PLONAS C18 (0.2 mm I.D. × 100 mm L., 2.7 m)
* Semi-micro data acquired with the 2.1 mm I.D. column which has the same chemical profile as an MC PLONAS column.
Mobile phase : A: Water, B: Methanol, gradient elution
Flow rate : 4 L/min Nexera Mikros: Trap and Elute Configuration
Temperature : 50 ºC
Injection volume : 1 L
MS detection : LCMS-8060, with Micro-ESI source, ESI (–) The trap and elute system can be constructed by using a dedicated piping kit (228-71751-42).

15 L Sample Loop Nexlock ™ ID 0.05 mm × 100 mm
Shim-pack MC PLONAS C8

1. All-trans Retinol (A) 6 SIL-40C XR Trap column
2. Epoxy-Menaquinone-4 (epoxy-MK-4) 5 A pump
3. Menaquinone-4 (MK-4) 1 Waste
4. α-Tocopherol (E) 4
5. Epoxy-Phylloquinone (epoxy-K1) 7 Mixer
6. Phylloquinone (K1) 2 1
7. Menaquinone-7 (MK-7)
3 6
3
2 4 5
B pump FCV-0206H
LC-40B X3
MS
Column : Shim-pack MC PLONAS C8 (0.2 mm I.D. × 100 mm L., 2.7 m) Analysis column
Mobile phase : A: Ammonium acetate aq. A pump
B: Ammonium acetate in methanol, gradient elution
Flow rate : 4 L/min Nexlock ID 0.05 mm × 600 mm
Temperature : 50 ºC
Injection volume: 0.2 L
0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 min MS detection : LCMS-8060, with Micro-ESI source, ESI (−) Nexlock ID 0.05 mm × 100 mm
20 pg on column B pump
* each peak is in full scale intensity
LC-Mikros

Nexera Mikros
10 Microflow Liquid Chromatography Mass Spectrometry System 11