Page 3 - Shimadzu iMLayer AERO

Imaging with High Reproducibility

• Humidity Control iMLayer AERO
Humidity does not impact matrix deposition because the atmosphere
within the spray chamber is replaced before pretreatment. Spraying can
be performed under more stable conditions than with a hand spray. Humidity has no impact.

N 2 purging

• Clog-Free Reagent Delivery
If the matrix clogs the nozzle tip, the spray becomes unstable, which can
lead to lower reproducibility. The rinsing mechanism allows for clog-free,
stable spraying, which enables high reproducibility in MALDI analyses.

Clog Rinse
solution

Both Sensitivity and Spatial Resolution Achieved

• Excellent Spray Performance
Sample plate
The standard protocol provides sensitivity on par with a hand spray
along with improved resolution, homogeneity and reproducibility. Body tissue Spraying
• Two-Step Vapor Deposition Sublimation
To acquire high spatial resolution (5 to 10 µm) images and increase Matrix
sensitivity, a two-step vapor deposition method can be implemented in
combination with the iMLayer matrix vapor deposition system has been Heating
developed.* This unique experiment can only be implemented using
Vapor Deposition Method Spray Method
Shimadzu sample preparation solutions.
* Patent No.: JP6153139 and JP6183779

Two-Step Vapor Deposition Allows for Very Fine Images with Minimal Blur Blur
Hand Spray
• Matrix : 9-AA
• Volume used : 200 µL

Two-Step Vapor Deposition
1 step: iMLayer (vapor deposition method)
• Film thickness : 1 µm
2 step: iMLayer AERO (spray method)
• Solution delivery volume : 120 µL/min
• Stage speed : 70 mm/sec
• Laminating layers : 4

iMLayer AERO
Automatic Sprayer for MALDI Imaging